Lignin triggers irreversible cellulase loss during pretreated lignocellulosic biomass saccharification
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چکیده
منابع مشابه
Lignin triggers irreversible cellulase loss during pretreated lignocellulosic biomass saccharification
BACKGROUND Non-productive binding of enzymes to lignin is thought to impede the saccharification efficiency of pretreated lignocellulosic biomass to fermentable sugars. Due to a lack of suitable analytical techniques that track binding of individual enzymes within complex protein mixtures and the difficulty in distinguishing the contribution of productive (binding to specific glycans) versus no...
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Mapping the location of bound cellulase enzymes provides information on the micro-scale distribution of amenable and recalcitrant sites in pretreated woody biomass for biofuel applications. The interaction of a fluorescently labelled cellulase enzyme cocktail with steam-exploded pine (SEW) was quantified using confocal microscopy. The spatial distribution of Dylight labelled cellulase was quant...
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ژورنال
عنوان ژورنال: Biotechnology for Biofuels
سال: 2014
ISSN: 1754-6834
DOI: 10.1186/s13068-014-0175-x